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Top : Immunology : ELISA : Cellular ELISA Protocol
Cellular ELISA Protocol
Author: Nanci Donacki
Source: Contributed by Nanci Donacki
Date Added: Tue May 14 2002
Date Modified: Wed Apr 28 2004

Formalin Fixed Cell Plates

1.
Trypsinize confluent flasks
2.
Pool and count cells
3.
Centrifuge at 1500 rpm for 10 minutes
4.
Resuspend to the appropriate concentration in complete medium
4 x 105 cells/ml for epithelial cells
2 x 105 cells/ml for fibroblast cells
5.
Add 100 ml/cell to 96 well culture plates.
6.
Incubate overnight at 37oC.
7.
Wash plates twice with PBS
8.
Add 125 ml/well 10% Buffered Formalin
9.
Fix for 15 minutes at room temperature
10.
Wash three times with di-H2O.
11.
Blot dry.
12.
Store at 2-8oC.

Reagents

1."